Mohammad Vafaee; Seyyed Reza Mohebi; Seyyed Reza Fatemi; Mohsen Vahedi; Khatoon Karimi; Akram Safaei; Maral Arkani; MohammadReza Zali
Volume 19, Issue 2 , May and June 2012, , Pages 109-115
Abstract
Background: Insulin-like growth factor 1 has an important role in cell proliferation and growth. Polymorphism in IGF-1 gene changes the level of this hormone and increases the risk of colorectal cancer. The aim of this study is to assess the incidence of IGF-1 polymorphism rs5742612 in Iranian population ...
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Background: Insulin-like growth factor 1 has an important role in cell proliferation and growth. Polymorphism in IGF-1 gene changes the level of this hormone and increases the risk of colorectal cancer. The aim of this study is to assess the incidence of IGF-1 polymorphism rs5742612 in Iranian population and to investigate the influence of this polymorphism in increasing the risk of colorectal cancer. Methods: Genotyping of IGF-1 gene was performed in a series of 110 colorectal cancer patients and 110 controls by using polymerase chain reaction and restriction fragment length polymorphism genotyping (PCR-RFLP) assays. We calculated odds ratio and confidence interval (CI) of IGF-1 genotypes to determine if these polymorphisms are associated with colorectal cancer. Result: No significant association was found between polymorphism of IGF-1 gene (rs5742612) and increased risk of colorectal cancer (p=0.92). The odds ratio for the heterozygous genotype CT versus the normal genotype CC was 1.3 (95% CI: 0.3-6.27) and the odds ratio for the mutant genotype TT versus the normal genotype CC was 0.0. The frequency of the mutant allele was 2.5%. Conclusion: These findings suggest that IGF-1 rs5742612 polymorphism is not associated with increased risk of colorectal cancer.